Collaborator ELK Biotechnology
Recombinant ProteinsISO 9001:2015
ABM100753

Recombinant Human VEGF165

ISO 9001|ABMIUM Verified™|Protein

Recombinant Human VEGF165 from ELK Biotechnology Read more ›

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Product Description

Recombinant Human VEGF165 is a research-use recombinant protein from ELK Biotechnology preparation representing VEGF165 supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Human Vascular Endothelial Growth Factor A is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.

Background

Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product typeRecombinant Proteins Target: VEGF165
Collaborator ELK Biotechnology
Partner model EPT029
Target VEGF165
Research areasMolecular Biology
Reactivity Product QC:C
Applications Functional assay
Assay type Recombinant protein
Purity >95% by SDS-PAGE
Endotoxin Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryPurityEndotoxinBioactivity
Product QC

Specifications

Target
VEGF165
Also known as
Vascular Endothelial Growth Factor Isoform 165; VEGF165
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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