Collaborator ELK Biotechnology
Recombinant ProteinsISO 9001:2015
ABM100737

Recombinant Human IL-7

ISO 9001|ABMIUM Verified™|Protein

Recombinant Human IL-7 from ELK Biotechnology Read more ›

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Product Description

Recombinant Human IL-7 is a research-use recombinant protein from ELK Biotechnology preparation representing IL-7 supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.

Background

Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT, which regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product typeRecombinant Proteins Target: IL-7
Collaborator ELK Biotechnology
Partner model EPT013
Target IL-7
Research areasMolecular Biology
Reactivity Product QC:C
Applications Functional assay
Assay type Recombinant protein
Purity >95% by SDS-PAGE
Endotoxin Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryPurityEndotoxinBioactivity
Product QC

Specifications

Target
IL-7
Also known as
Interleukin-7; IL-7; IL7
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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