Collaborator ELK Biotechnology
Recombinant ProteinsISO 9001:2015
ABM100732

Recombinant K. pneumoniae NEO

ISO 9001|ABMIUM Verified™|Protein

Recombinant K. pneumoniae NEO from ELK Biotechnology Read more ›

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Product Description

Recombinant K. pneumoniae NEO is a research-use recombinant protein from ELK Biotechnology preparation representing Recombinant K. pneumoniae NEO supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Klebsiella Pneumoniae Aminoglycoside 3'-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.

Background

Aminoglycoside 3'-phosphotransferase (APH(3')), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH(3') is primarily found in certain species of gram-positive bacteria.

Handling information

Delivery condition The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product typeRecombinant Proteins Target: Recombinant K. pneumoniae NEO
Collaborator ELK Biotechnology
Partner model EPT008
Target Recombinant K. pneumoniae NEO
Research areasMolecular Biology
Reactivity Product QC:C
Applications Protein research
Assay type Recombinant protein
Purity >95% by SDS-PAGE
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryPurityEndotoxinBioactivity
Product QC

Specifications

Target
Recombinant K. pneumoniae NEO
Also known as
Aminoglycoside 3'-phosphotransferase; APH(3')-II; APH(3')II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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