Collaborator ELK Biotechnology
Recombinant ProteinsISO 9001:2015
ABM100728

Recombinant Human EGF

ISO 9001|ABMIUM Verified™|Protein

Recombinant Human EGF from ELK Biotechnology Read more ›

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Product Description

Recombinant Human EGF is a research-use recombinant protein from ELK Biotechnology preparation representing EGF supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.

Background

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product typeRecombinant Proteins Target: EGF
Collaborator ELK Biotechnology
Partner model EPT004
Target EGF
Research areasMolecular Biology
Reactivity Product QC:C
Applications Functional assay
Assay type Recombinant protein
Purity >95% by SDS-PAGE
Endotoxin Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryPurityEndotoxinBioactivity
Product QC

Specifications

Target
EGF
Also known as
Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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