Recombinant Protein ISO 9001|ABMIUM Verified™
ABM100727

Recombinant 2019-nCoV Guanine-N7_meth (N-6His)

ISO 9001|ABMIUM Verified™|Protein

Recombinant 2019-nCoV Guanine-N7_meth (N-6His) from ELK Biotechnology Read more ›

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Product Description

Recombinant 2019-nCoV Guanine-N7_meth (N-6His) is an ELK Biotechnology recombinant protein for research use.

Description

Recombinant 2019-nCoV Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus.

Background

The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.

Handling information

Delivery condition The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product type Recombinant Protein Target: Recombinant 2019-nCoV Guanine-N7_meth
Target Recombinant 2019-nCoV Guanine-N7_meth
Also known as SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
Purity Greater than 85% as determined by reducing SDS-PAGE.
Form / buffer Lyophilized protein
Research area Infectious Diseases, Molecular Biology
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1 specification
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpecificationPURITYENDOTOXINBIOACTIVITY
Product QC

Specifications

Target
Recombinant 2019-nCoV Guanine-N7_meth
Also known as
SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
Purity
Greater than 85% as determined by reducing SDS-PAGE.
Form / buffer
Lyophilized protein

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Storage conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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