Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92604

Collagen I (4B5) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Collagen I (4B5) Mouse mAb from ELK Biotechnology Read more ›

Collagen I (4B5) Mouse mAb
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Collagen I (4B5) Mouse mAb

Product Description

Collagen I (4B5) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Collagen I (4B5) in IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Type 1 collagen is a trimeric complex comprised of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and posttranslational modification.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Collagen I (EM1387)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Collagen I (EM1387)Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: Collagen I (4B5)
Collaborator ELK Biotechnology
Partner model EM1387
Target Collagen I (4B5)
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:100-200
Mouse
Rat

Specifications

Target
Collagen I (4B5)
Also known as
Alpha 1 type I collagen, COL1A1, COL1A3
Concentration
1mg/mL
Working concentration
IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IHC
Immunogen
Synthetic Peptide
Observed band
139kDa
Cellular localization
Secreted,extracellular space,extracellular matrix
Dilution range
IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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