Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92597

Collagen II (8F6) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Collagen II (8F6) Mouse mAb from ELK Biotechnology Read more ›

Collagen II (8F6) Mouse mAb
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Collagen II (8F6) Mouse mAb

Product Description

Collagen II (8F6) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Collagen II (8F6) in IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Collagen II (EM1372)Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: Collagen II (8F6)
Collaborator ELK Biotechnology
Partner model EM1372
Target Collagen II (8F6)
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:100-200
Mouse
Rat

Specifications

Target
Collagen II (8F6)
Also known as
Alpha 1 type II collagen , AOM, Col2a1 antibody
Concentration
1mg/mL
Working concentration
IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IHC
Immunogen
Synthetic Peptide
Observed band
142kDa
Cellular localization
Secreted,extracellular space,extracellular matrix
Dilution range
IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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