ABM92594
HP-1α (5E3) Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
HP-1α (5E3) Mouse mAb from ELK Biotechnology Read more ›
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Product Description
HP-1α (5E3) Mouse mAb is an ELK Biotechnology antibody for research workflows involving HP-1α (5E3).
Background
Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin.
Additional antibody information
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α（EM1366）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1α （EM1366）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 α （EM1366）Mouse mAb diluted at 1:200 | Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1α （EM1366）Mouse mAb diluted at 1:1000. |
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Key Facts
Product type
Primary Antibody›
Target: HP-1α (5E3)
| Target | HP-1α (5E3) |
| Also known as | Antigen p25, CBX5, HEL25, Heterochromatin protein 1 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:500-1,000 IHC 1:100-200 |
| Species reactivity | Human, Mouse, Rat |
| Observed band | 22kDa |
| Cellular localization | Cytoplasm, Membrane, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-1,000 IHC 1:100-200 |
| Immunogen | Recombinant Protein |
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Citations
ISO 9001
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-1 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
HP-1α (5E3)
Also known as
Antigen p25, CBX5, HEL25, Heterochromatin protein 1
Concentration
1mg/mL
Working concentration
WB 1:500-1,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Mouse, Rat
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
22kDa
Cellular localization
Cytoplasm, Membrane, Nucleus
Dilution range
WB 1:500-1,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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