Flag-Tag (1C1) Mouse mAb
Flag-Tag (1C1) Mouse mAb from ELK Biotechnology Read more ›
Product Description
Flag-Tag (1C1) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Flag-Tag (1C1).
Background
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Additional antibody information
| Validation evidence captions | Western blot analysis of Flag Recombinant Protein using Flag （EM1335）Mouse mAb diluted at 1:10000. |
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Key Facts
| Target | Flag-Tag (1C1) |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 |
| Species reactivity | ALL |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:5,000 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| ALL |
| Species | Dilution | Notes |
|---|---|---|
| ALL | WB 1:5 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.