ABM92575
Smad3 (4C9) Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Smad3 (4C9) Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Smad3 (4C9) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Smad3 (4C9).
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Mouse Brain Tissue Lysate, 2) Rat Brain Tissue Lysate using Smad3（ EM1315）Mouse mAb diluted at 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3（EM1315）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Smad3（EM1315）Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: Smad3 (4C9)
| Target | Smad3 (4C9) |
| Also known as | hMAD3 antibody, HSPC193 antibody, LDS1C antibody, MADH3 antibody, JV15 2 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 52kDa |
| Cellular localization | Cytoplasm, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Smad3 (4C9)
Also known as
hMAD3 antibody, HSPC193 antibody, LDS1C antibody, MADH3 antibody, JV15 2 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
52kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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