ABM92570
Beclin-1 (5C2) Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Beclin-1 (5C2) Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Beclin-1 (5C2) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Beclin-1 (5C2).
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions. One of the proteins critical to this process is Beclin-1.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) 293T Cell Lysate, 2) C2C12 Cell Lysate, 3) Rat Brain Tissue Lysate using Beclin-1（ EM1304 ）Mouse mAb diluted at 1:2000. | Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Beclin-1（EM1304）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Beclin-1（EM1304）Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: Beclin-1 (5C2)
| Target | Beclin-1 (5C2) |
| Also known as | ATG6, Bcl-2 interacting protein beclin, BECN1, Coiled coil myosin like BCL2 interacting protein |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 60kDa |
| Cellular localization | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Beclin-1 (5C2)
Also known as
ATG6, Bcl-2 interacting protein beclin, BECN1, Coiled coil myosin like BCL2 interacting protein
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
60kDa
Cellular localization
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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