Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92567

Cyclin B1 (1A5) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Cyclin B1 (1A5) Mouse mAb from ELK Biotechnology Read more ›

Cyclin B1 (1A5) Mouse mAb
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Cyclin B1 (1A5) Mouse mAb

Product Description

Cyclin B1 (1A5) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Cyclin B1 (1A5) in IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (Cdk1) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase of the cell cycle.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Cyclin B1(EM1299)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Cyclin B1( EM1299 )Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: Cyclin B1 (1A5)
Collaborator ELK Biotechnology
Partner model EM1299
Target Cyclin B1 (1A5)
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:200-500
Host Mouse
Clonality Monoclonal
Isotype IgG
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:200-500
Rat
Mouse

Specifications

Target
Cyclin B1 (1A5)
Also known as
ATG8E, MAP1LC3A,autophage-related protein LC3A
Concentration
1mg/mL
Working concentration
IHC 1:200-500
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHC
Immunogen
Synthetic Peptide
Observed band
55kDa
Cellular localization
Cytoplasm, Cytoskeleton, Nucleus
Dilution range
IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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