ABM92566
LC3A (5G10) Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
LC3A (5G10) Mouse mAb from ELK Biotechnology Read more ›
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Product Description
LC3A (5G10) Mouse mAb is an ELK Biotechnology antibody for research workflows involving LC3A (5G10).
Background
Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using LC3A （ EM1298 ）Mouse mAb diluted at 1:1000. | Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A（EM1298）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A（EM1298 ）Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: LC3A (5G10)
| Target | LC3A (5G10) |
| Also known as | ATG8E, MAP1LC3A,autophage-related protein LC3A |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 14,16kDa |
| Cellular localization | Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
LC3A (5G10)
Also known as
ATG8E, MAP1LC3A,autophage-related protein LC3A
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
14,16kDa
Cellular localization
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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