LC3A (5G10) Mouse mAb from ELK Biotechnology Read more ›
LC3A (5G10) Mouse mAb is a research-use antibody from ELK Biotechnology for studying LC3A (5G10) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
| Validation evidence captions | Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using LC3A （ EM1298 ）Mouse mAb diluted at 1:1000. | Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A（EM1298）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A（EM1298 ）Mouse mAb diluted at 1:200. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1298 |
| Target | LC3A (5G10) |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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