Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92558

AKT (1H2) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

AKT (1H2) Mouse mAb from ELK Biotechnology Read more ›

AKT (1H2) Mouse mAb
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AKT (1H2) Mouse mAb

Product Description

AKT (1H2) Mouse mAb is a research-use antibody from ELK Biotechnology for studying AKT (1H2) in WB, IHC workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Akt, is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth.

Additional antibody information

Validation evidence captions Western blot analysis of PC3 Cell Lysateusing Akt(EM1271)Mouse mAb diluted at 1:1000. | Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Akt(EM1271)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Akt(EM1271)Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: AKT (1H2)
Collaborator ELK Biotechnology
Partner model EM1271
Target AKT (1H2)
Research areasAntibody Research
Reactivity Human
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
AKT (1H2)
Also known as
c Akt antibody, MGC9965 antibody, Protein kinase B antibody, RAC antibody, PRKBA antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
60kDa
Cellular localization
Cytoplasm,Nucleus,Cell membrane
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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