Phospho-Akt (S473) (6F8) Mouse mAb
Phospho-Akt (S473) (6F8) Mouse mAb from ELK Biotechnology Read more ›
Product Description
Phospho-Akt (S473) (6F8) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Phospho-Akt (S473) (6F8).
Background
Akt, is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth.
Additional antibody information
| Validation evidence captions | Western blot analysis of PC3 Cell Lysateusing Phospho-Akt Ser473（EM1269）Mouse mAb diluted at 1:1000. | Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473（EM1269）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Phospho-Akt Ser473（EM1269）Mouse mAb diluted at 1:200. |
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Key Facts
| Target | Phospho-Akt (S473) (6F8) |
| Also known as | c Akt antibody, MGC9965 antibody, Protein kinase B antibody, RAC antibody, PRKBA antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human |
| Observed band | 60kDa |
| Cellular localization | Cytoplasm,Nucleus,Cell membrane |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.