Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92556

Bax (6F11) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Bax (6F11) Mouse mAb from ELK Biotechnology Read more ›

Bax (6F11) Mouse mAb
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Bax (6F11) Mouse mAb

Product Description

Bax (6F11) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Bax (6F11) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysateusing Bax(EM1268)Mouse mAb diluted at 1:1000. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax(EM1268)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax(EM1268)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax(EM1268)Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: Bax (6F11)
Collaborator ELK Biotechnology
Partner model EM1268
Target Bax (6F11)
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Bax (6F11)
Also known as
Apoptosis regulator bax, BAXA, BCL2 associated X protein
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
20kDa
Cellular localization
Cytoplasm, Membrane, Mitochondrion
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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