Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92546

HIF-1 β/ARNT Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

HIF-1 β/ARNT Mouse mAb from ELK Biotechnology Read more ›

HIF-1 β/ARNT Mouse mAb
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HIF-1 β/ARNT Mouse mAb

Product Description

HIF-1 β/ARNT Mouse mAb is a research-use antibody from ELK Biotechnology for studying HIF-1 β/ARNT in WB, IHC workflows with listed reactivity for Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Hypoxia-inducible factors (HIFs) are transcription factors that respond to changes in available oxygen in the cellular environment, to be specific, to decreases in oxygen, or hypoxia.This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding.

Additional antibody information

Validation evidence captions Western blot analysis of Mouse Brain Tissue with HIF-1 β/ARNT Mouse mAb EM1242 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse BrainTissue using HIF-1 β/ARNT (EM1242)Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: HIF-1 β/ARNT
Collaborator ELK Biotechnology
Partner model EM1242
Target HIF-1 β/ARNT
Research areasAntibody Research
Reactivity Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Mouse WB 1:1
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
HIF-1 β/ARNT
Also known as
HIF 1 beta, ARNT, Tango, hypoxia inducible factor 1 beta, Nrnt, Dioxin receptor, bHLH2e
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
87kDa
Cellular localization
Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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