Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92545

Galectin-3 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Galectin-3 Mouse mAb from ELK Biotechnology Read more ›

Galectin-3 Mouse mAb
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Galectin-3 Mouse mAb

Product Description

Galectin-3 Mouse mAb is a research-use antibody from ELK Biotechnology for studying Galectin-3 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

Additional antibody information

Validation evidence captions Western blot analysis of 1)MCF7, 2) 3T3 with Galectin-3 Mouse mAb EM1237 diluted at 1:2,000.

Key Facts

Product typePrimary Antibody Target: Galectin-3
Collaborator ELK Biotechnology
Partner model EM1237
Target Galectin-3
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200 ELISA 10,000-50,000
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Galectin-3
Also known as
35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200 ELISA 10,000-50,000
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIHCELISA
Immunogen
Protein
Observed band
26kDa
Cellular localization
Cytoplasm, Nucleus, Secreted, Spliceosome
Dilution range
WB 1:1,000-2,000 IHC 1:100-200 ELISA 10,000-50,000

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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