ABM92541
AMPK α1 Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
AMPK α1 Mouse mAb from ELK Biotechnology Read more ›
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Product Description
AMPK α1 Mouse mAb is an ELK Biotechnology antibody for research workflows involving AMPK α1.
Background
AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3).
Additional antibody information
| Validation evidence captions | Western blot analysis of 1)Hela, 2) 293T, 3)3T3, 4) PC12 with AMPK a1 Mouse mAb EM1225 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse ColonTissue using AMPK a1 (EM1225) Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: AMPK α1
| Target | AMPK α1 |
| Also known as | AAPK1, 5 AMP activated protein kinase alpha 1 catalytic subunit, PRKAA1, SNF1A, MGC33776 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 63kDa |
| Cellular localization | Cytoplasm, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
ISO 9001
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
AMPK α1
Also known as
AAPK1, 5 AMP activated protein kinase alpha 1 catalytic subunit, PRKAA1, SNF1A, MGC33776
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
63kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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