Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92533

GRP78/Bip Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

GRP78/Bip Mouse mAb from ELK Biotechnology Read more ›

GRP78/Bip Mouse mAb 50µL/100µL
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GRP78/Bip Mouse mAb 50µL/100µL

Product Description

GRP78/Bip Mouse mAb is a research-use antibody from ELK Biotechnology for studying GRP78/Bip in WB, IHC workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Belongs to the heat shock protein 70 family. When protein folding is disturbed inside ER, BiP synthesis is increased.

Additional antibody information

Validation evidence captions Western blot analysis of 1)Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb EM1210 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Colon Caricnoma using GRP78/Bip(EM1210)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using GRP78/Bip(EM1210)Mouse mAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: GRP78/Bip
Collaborator ELK Biotechnology
Partner model EM1210
Target GRP78/Bip
Research areasAntibody Research
Reactivity Human,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
GRP78/Bip
Also known as
HSPA5, GRP78,MIF2, BIP, Heat shock 70 kda protein 5
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
78kDa
Cellular localization
Endoplasmic reticulum lumen, Melanosome,Cytoplasm.
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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