ABM92517
Active Caspase 3 Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Active Caspase 3 Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Active Caspase 3 Mouse mAb is an ELK Biotechnology antibody for research workflows involving Active Caspase 3.
Background
Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2)3T3, 3)Rat Brain Tissue with Active Caspase 3 Mouse mAb EM1188 diluted at 1:1,000. | Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase 3 （EM1188）Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: Active Caspase 3
| Target | Active Caspase 3 |
| Also known as | APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:500-1,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 17kDa |
| Cellular localization | Cytoplasm |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-1,000 IHC 1:100-200 |
| Immunogen | Recombinant Protein |
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Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Active Caspase 3
Also known as
APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody
Concentration
1mg/mL
Working concentration
WB 1:500-1,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
17kDa
Cellular localization
Cytoplasm
Dilution range
WB 1:500-1,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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