ABM92495
eNOS Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
eNOS Mouse mAb from ELK Biotechnology Read more ›
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Product Description
eNOS Mouse mAb is an ELK Biotechnology antibody for research workflows involving eNOS.
Background
Nitric oxide synthases (EC 1.14.13.39) (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), generates NO in blood vessels and is involved with regulating vascular function.
Additional antibody information
| Validation evidence captions | Western blot analysis of Rat Heart Tissue with eNOS (Mix-mA ™) Mouse mAb EM1165 diluted at 1:1,000. | Immunohistochemical analysis of paraffin-embedded Mouse Colon Tissue using eNOS （EM1165）Mouse mAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: eNOS
| Target | eNOS |
| Also known as | endothelial nitric oxidase synthase antibody, NOS 3 antibody, NOS III antibody, EC-NOS antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:500-2,000 IHC 1：200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 130kDa |
| Cellular localization | Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2,000 IHC 1：200-500 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
eNOS
Also known as
endothelial nitric oxidase synthase antibody, NOS 3 antibody, NOS III antibody, EC-NOS antibody
Concentration
1mg/mL
Working concentration
WB 1:500-2,000 IHC 1：200-500
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
130kDa
Cellular localization
Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane
Dilution range
WB 1:500-2,000 IHC 1：200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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