ABM92489
Vimentin Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Vimentin Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Vimentin Mouse mAb is an ELK Biotechnology antibody for research workflows involving Vimentin.
Background
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules.Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2)Mouse Brain Tissue , 3) Rat Brain tissue with EM1159 diluted at 1:2,000. | IF analysis of Hela with EM1159(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded human breast caricnoma using Vimentin （EM1159）Mouse mAb diluted at 1:500. | Western blot analysis of extracts from MCF-7 using Vimentin diluted at 1:3,000 |
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Key Facts
Product type
Primary Antibody›
Target: Vimentin
| Target | Vimentin |
| Also known as | FLJ36605 antibody, VIM antibody, VIME antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 50-57kDa |
| Cellular localization | Cytoplasm |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
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Citations
ISO 9001
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
Vimentin
Also known as
FLJ36605 antibody, VIM antibody, VIME antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHCIF
Immunogen
Recombinant Protein
Observed band
50-57kDa
Cellular localization
Cytoplasm
Dilution range
WB 1:1,000-3,000 IF 1:100-200 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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