Cleaved PARP Mouse mAb from ELK Biotechnology Read more ›
Cleaved PARP Mouse mAb is a research-use antibody from ELK Biotechnology for studying Cleaved PARP in WB, IHC workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.
| Validation evidence captions | Western blot analysis of Jurkat with EM1145 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded human Tonsil Tissue using Cleaved PARP （EM1145）Mouse mAb diluted at 1:500. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1145 |
| Target | Cleaved PARP |
| Research areas | Antibody Research |
| Reactivity | Human |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:2,000-5,000 IHC 1:200-500 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:2 | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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