Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92475

Fibronectin Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Fibronectin Mouse mAb from ELK Biotechnology Read more ›

Fibronectin Mouse mAb
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Fibronectin Mouse mAb

Product Description

Fibronectin Mouse mAb is a research-use antibody from ELK Biotechnology for studying Fibronectin in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Similar to integrins, fibronectin binds extracellular matrix components such as collagen.Fibronectin exists as a protein dimer, consisting of two nearly identical monomers linked by a pair of disulfide bonds. The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms.

Additional antibody information

Validation evidence captions Western blot analysis of Hela with EM1137 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Fibronectin(EM1137)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Fibronectin
Collaborator ELK Biotechnology
Partner model EM1137
Target Fibronectin
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Fibronectin
Also known as
CIG, Cold insoluble globulin, fibronectin 1, FN, fN1, FNZ, GFND, GFND2, UgI-Y3
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
285kDa
Cellular localization
Secreted, extracellular space, extracellular matrix
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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