eIF4A1 Mouse mAb from ELK Biotechnology Read more ›
eIF4A1 Mouse mAb is a research-use antibody from ELK Biotechnology for studying eIF4A1 in WB, IHC, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA - this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.
| Validation evidence captions | Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000. | IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（EM1136）Mouse mAb diluted at 1:200. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1136 |
| Target | eIF4A1 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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