ABM92474
eIF4A1 Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
eIF4A1 Mouse mAb from ELK Biotechnology Read more ›
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Product Description
eIF4A1 Mouse mAb is an ELK Biotechnology antibody for research workflows involving eIF4A1.
Background
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA - this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000. | IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（EM1136）Mouse mAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: eIF4A1
| Target | eIF4A1 |
| Also known as | DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 48kDa |
| Cellular localization | Cytosol,Extracellular region or secreted |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
eIF4A1
Also known as
DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A
Concentration
1mg/mL
Working concentration
WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHCIF
Immunogen
Synthetic Peptide
Observed band
48kDa
Cellular localization
Cytosol,Extracellular region or secreted
Dilution range
WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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