EGFR Mouse mAb from ELK Biotechnology Read more ›
EGFR Mouse mAb is a research-use antibody from ELK Biotechnology for studying EGFR in WB, IHC, IF, IP workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.
| Validation evidence captions | Western blot analysis of 1) A431 ,2) Hela, with EM1134 diluted at 1:2,000. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1134 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific(S003), 1:5,000 | IF analysis of Hela with EM1134(Left) and DAPI (Right) diluted at 1:100. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1134 |
| Target | EGFR |
| Research areas | Antibody Research |
| Reactivity | Human |
| Applications | WB,IHC,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-3,000 IP 1:200 IF 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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