EGFR Mouse mAb
EGFR Mouse mAb from ELK Biotechnology Read more ›
Product Description
EGFR Mouse mAb is an ELK Biotechnology antibody for research workflows involving EGFR.
Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) A431 ,2) Hela, with EM1134 diluted at 1:2,000. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1134 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Heavy Chain Specific(S003), 1:5,000 | IF analysis of Hela with EM1134(Left) and DAPI (Right) diluted at 1:100. |
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Key Facts
| Target | EGFR |
| Also known as | Epidermal Growth Factor Receptor ;ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IP 1:200 IF 1:100-200 |
| Species reactivity | Human |
| Observed band | 170kDa |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFIP |
| Dilution range | WB 1:1,000-3,000 IP 1:200 IF 1:100-200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.