PARP Mouse mAb
PARP Mouse mAb from ELK Biotechnology Read more ›
Product Description
PARP Mouse mAb is an ELK Biotechnology antibody for research workflows involving PARP.
Background
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat with EM1131 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded human Tonsil Tissue using PARP （EM1131）Mouse mAb diluted at 1:500. |
|---|
Key Facts
| Target | PARP |
| Also known as | PARP-1, Poly(ADP ribose) polymerase 1, sPARP1,ADPRT1, ADP ribosyltransferase NAD(+) |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IHC 1:200-500 |
| Species reactivity | Human |
| Observed band | 116kDa |
| Cellular localization | Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-3,000 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.