ABM92456
Histone H3(tri methyl K9) Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Histone H3(tri methyl K9) Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Histone H3(tri methyl K9) Mouse mAb is an ELK Biotechnology antibody for research workflows involving Histone H3(tri methyl K9).
Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Additional antibody information
| Validation evidence captions | Western blot analysis of Hela with EM1109 diluted at 1) 1:2,000 2) 1:5,000 | Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7 with EM1109 diluted at 1:2000. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1109 1:1,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000 | Immunohistochemical analysis of paraffin-embedded Human Clolon using Histone H3(tri methyl K9)（EM1109）Mouse mAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: Histone H3(tri methyl K9)
| Target | Histone H3(tri methyl K9) |
| Also known as | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IP 1:200 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 15kDa |
| Cellular localization | Nucleus,Chromosome |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-3,000 IP 1:200 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Histone H3(tri methyl K9)
Also known as
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-3,000 IP 1:200 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
15kDa
Cellular localization
Nucleus,Chromosome
Dilution range
WB 1:1,000-3,000 IP 1:200 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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