Histone H3(di methyl K27) Mouse mAb from ELK Biotechnology Read more ›
Histone H3(di methyl K27) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Histone H3(di methyl K27) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
| Validation evidence captions | Western blot analysis of Hela withHistone H3 (di methyl K27) (1E10) Mouse MAb diluted at 1) 1:1,000 2) 1:3,000 | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1101 1:2,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000 | Immunohistochemical analysis of paraffin-embedded Human Skin using Histone H3(di methyl K27)（EM1101）Mouse mAb diluted at 1:500. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1101 |
| Target | Histone H3(di methyl K27) |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-3,000 IP 1:200 IHC 1:200-500 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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