Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92445

Histone H3 (di methyl K79) Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Histone H3 (di methyl K79) Mouse mAb from ELK Biotechnology Read more ›

Histone H3 (di methyl K79) Mouse mAb
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Histone H3 (di methyl K79) Mouse mAb

Product Description

Histone H3 (di methyl K79) Mouse mAb is a research-use antibody from ELK Biotechnology for studying Histone H3 (di methyl K79) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Additional antibody information

Validation evidence captions Western blot analysis of Hela with Histone H3 (di methyl K79) (Q7) Mouse mAb diluted at 1:2,000 | Western blot analysis of 293T with Histone H3 (di methyl K79) (Q7) Mouse mAb diluted at 1:2,000 | Western blot analysis of 1) Rab Spleen, 2) HepG2, 3) Mouse Spleen tissue with Histone H3 (di methyl K79) (Q7) Mouse mAb diluted at 1:2,000 | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H3(di methyl K79)(EM1087)Mouse mAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Histone H3 (di methyl K79)
Collaborator ELK Biotechnology
Partner model EM1087
Target Histone H3 (di methyl K79)
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2,000 IHC 1:200-500
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Histone H3 (di methyl K79)
Also known as
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Concentration
1mg/mL
Working concentration
WB 1:500-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
15kDa
Cellular localization
Nucleus,Chromosome
Dilution range
WB 1:500-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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