Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92441

IDE/Insulin Degrading Enzyme Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

IDE/Insulin Degrading Enzyme Mouse mAb from ELK Biotechnology Read more ›

IDE/Insulin Degrading Enzyme Mouse mAb
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IDE/Insulin Degrading Enzyme Mouse mAb

Product Description

IDE/Insulin Degrading Enzyme Mouse mAb is a research-use antibody from ELK Biotechnology for studying IDE/Insulin Degrading Enzyme in WB, IHC workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Insulin Degrading Enzyme (IDE) is a large zinc-binding protease of the M16A metalloprotease subfamily known to cleave multiple short polypeptides that vary considerably in sequence. IDE was first identified by its ability to degrade the B chain of the hormone insulin. This activity was observed over fifty years ago, though the enzyme specifically responsible for B chain cleavage was identified more recently.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2) HepG2,with IDE Mouse mAb diluted at 1:2,000. | Western blot analysis of Hela, HepG2,with IDE Mouse mAb diluted at 1:2,000.

Key Facts

Product typePrimary Antibody Target: IDE/Insulin Degrading Enzyme
Collaborator ELK Biotechnology
Partner model EM1083
Target IDE/Insulin Degrading Enzyme
Research areasAntibody Research
Reactivity Human
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000 IHC 1:200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
IDE/Insulin Degrading Enzyme
Also known as
Abeta degrading protease, insulin degrading enzyme, Insulin protease, Insulinase, INSULYSIN
Concentration
1mg/mL
Working concentration
WB 1:1,000 IHC 1:200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
118kDa
Cellular localization
Cell membrane, Cytoplasm, Membrane, Secreted
Dilution range
WB 1:1,000 IHC 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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