Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92437

ErbB-2/HER-2 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

ErbB-2/HER-2 Mouse mAb from ELK Biotechnology Read more ›

ErbB-2/HER-2 Mouse mAb 50µL/100µL
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ErbB-2/HER-2 Mouse mAb 50µL/100µL

Product Description

ErbB-2/HER-2 Mouse mAb is a research-use antibody from ELK Biotechnology for studying ErbB-2/HER-2 in WB, IHC workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2) 293, 3) Jurkat,with ErbB-2 Mouse mAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using ErbB-2/HER-2(EM1079)Mouse mAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: ErbB-2/HER-2
Collaborator ELK Biotechnology
Partner model EM1079
Target ErbB-2/HER-2
Research areasAntibody Research
Reactivity Human
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000 IHC 1:200-500
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
ErbB-2/HER-2
Also known as
CD340, HER 2/neu, HER2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1
Concentration
1mg/mL
Working concentration
WB 1:1,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
138kDa
Cellular localization
Cytoplasm. Nucleus and Cell membrane
Dilution range
WB 1:1,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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