ABM92435
Caspase 9 Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Caspase 9 Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Caspase 9 Mouse mAb is an ELK Biotechnology antibody for research workflows involving Caspase 9.
Background
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Additional antibody information
| Validation evidence captions | Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1）1:2,000 2）1:5,000 | Western blot analysis of 1）Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000 | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100. |
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Key Facts
Product type
Primary Antibody›
Target: Caspase 9
| Target | Caspase 9 |
| Also known as | APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-5,000 IP 1:200 IF 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 47（51）kDa |
| Cellular localization | Cytosol,Mitochondrion,Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIFIP |
| Dilution range | WB 1:1,000-5,000 IP 1:200 IF 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IP
| Species | Dilution | Notes |
|---|
Specifications
Target
Caspase 9
Also known as
APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-5,000 IP 1:200 IF 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIFIP
Immunogen
Synthetic Peptide
Observed band
47（51）kDa
Cellular localization
Cytosol,Mitochondrion,Nucleus
Dilution range
WB 1:1,000-5,000 IP 1:200 IF 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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