Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92435

Caspase 9 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Caspase 9 Mouse mAb from ELK Biotechnology Read more ›

Caspase 9 Mouse mAb
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Caspase 9 Mouse mAb

Product Description

Caspase 9 Mouse mAb is a research-use antibody from ELK Biotechnology for studying Caspase 9 in WB, IF, IP workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Additional antibody information

Validation evidence captions Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1)1:2,000 2)1:5,000 | Western blot analysis of 1)Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000 | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100.

Key Facts

Product typePrimary Antibody Target: Caspase 9
Collaborator ELK Biotechnology
Partner model EM1077
Target Caspase 9
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IF,IP
ConjugationUnconjugated
Dilution rangeWB 1:1,000-5,000 IP 1:200 IF 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ IP
Species Dilution Notes

Specifications

Target
Caspase 9
Also known as
APAF 3 antibody, Apoptosis related cysteine peptidase antibody, Apoptotic protease-activating factor 3 antibody, CASP 9 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-5,000 IP 1:200 IF 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIFIP
Immunogen
Synthetic Peptide
Observed band
47(51)kDa
Cellular localization
Cytosol,Mitochondrion,Nucleus
Dilution range
WB 1:1,000-5,000 IP 1:200 IF 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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