Caspase 9 Mouse mAb from ELK Biotechnology Read more ›
Caspase 9 Mouse mAb is a research-use antibody from ELK Biotechnology for studying Caspase 9 in WB, IF, IP workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Caspase-9 is an initiator caspase, encoded by the CASP9 gene.Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
| Validation evidence captions | Western blot analysis of Hela with Caspase 9 Mouse mAb diluted at 1)1:2,000 2)1:5,000 | Western blot analysis of 1)Jurkat, 2) HepG2, 3) Mouse Heart, 4) Rat Heart with Caspase 9 Mouse mAb diluted at 1:3,000 | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1077 1:1,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | IF analysis of Hela with EM1077(Left) and DAPI (Right) diluted at 1:100. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1077 |
| Target | Caspase 9 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-5,000 IP 1:200 IF 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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