CA IX/Carbonic Anhydrase IX Mouse mAb
CA IX/Carbonic Anhydrase IX Mouse mAb from ELK Biotechnology Read more ›
Product Description
CA IX/Carbonic Anhydrase IX Mouse mAb is an ELK Biotechnology antibody for research workflows involving CA IX/Carbonic Anhydrase IX.
Background
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) 293T, with CA IX Mouse mAb diluted at 1:5,000. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1076 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using CA IX/Carbonic Anhydrase IX（EM1076）Mouse mAb diluted at 1:200. |
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Key Facts
| Target | CA IX/Carbonic Anhydrase IX |
| Also known as | CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:3,000 IP 1:200 IHC 1:100-200 |
| Species reactivity | Human |
| Observed band | 35-38kDa |
| Cellular localization | Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIP |
| Dilution range | WB 1:3,000 IP 1:200 IHC 1:100-200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:3 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.