CA IX/Carbonic Anhydrase IX Mouse mAb from ELK Biotechnology Read more ›
CA IX/Carbonic Anhydrase IX Mouse mAb is a research-use antibody from ELK Biotechnology for studying CA IX/Carbonic Anhydrase IX in WB, IHC, IP workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors.
| Validation evidence captions | Western blot analysis of 1) Hela, 2) 293T, with CA IX Mouse mAb diluted at 1:5,000. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1076 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using CA IX/Carbonic Anhydrase IX（EM1076）Mouse mAb diluted at 1:200. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1076 |
| Target | CA IX/Carbonic Anhydrase IX |
| Research areas | Antibody Research |
| Reactivity | Human |
| Applications | WB,IHC,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:3,000 IP 1:200 IHC 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:3 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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