FH/Fumarase Mouse mAb
FH/Fumarase Mouse mAb from ELK Biotechnology Read more ›
Product Description
FH/Fumarase Mouse mAb is an ELK Biotechnology antibody for research workflows involving FH/Fumarase.
Background
Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, with FH Mouse mAb diluted at 1:3,000. | Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, with FH Mouse mAb diluted at 1:3,000. | IF analysis of Hela with EM1073 diluted at 1:100. |
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Key Facts
| Target | FH/Fumarase |
| Also known as | Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:3,000 IF 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 50kDa |
| Cellular localization | Cytoplasm and Mitochondrion. |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIF |
| Dilution range | WB 1:3,000 IF 1:100-200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:3 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.