FH/Fumarase Mouse mAb from ELK Biotechnology Read more ›
FH/Fumarase Mouse mAb is a research-use antibody from ELK Biotechnology for studying FH/Fumarase in WB, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety.
| Validation evidence captions | Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, with FH Mouse mAb diluted at 1:3,000. | Western blot analysis of 1) Mouse Brain tissue, 2) Rat Brain tissue, with FH Mouse mAb diluted at 1:3,000. | IF analysis of Hela with EM1073 diluted at 1:100. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1073 |
| Target | FH/Fumarase |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF |
| Conjugation | Unconjugated |
| Dilution range | WB 1:3,000 IF 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:3 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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