Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92424

NSE Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

NSE Mouse mAb from ELK Biotechnology Read more ›

NSE Mouse mAb
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NSE Mouse mAb

Product Description

NSE Mouse mAb is a research-use antibody from ELK Biotechnology for studying NSE in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates with NSE mAb diluted at 1:3,000. | IHC staining of Human small cell carcinoma of lung tissue with NSEmouse mAb(13E2) diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: NSE
Collaborator ELK Biotechnology
Partner model EM1066
Target NSE
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:2,000 IHC 1:200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:2
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
NSE
Also known as
ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase
Concentration
1mg/mL
Working concentration
WB 1:2,000 IHC 1:200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
47kDa
Cellular localization
Cytoplasm. Cell membrane.
Dilution range
WB 1:2,000 IHC 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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