NSE Mouse mAb
NSE Mouse mAb from ELK Biotechnology Read more ›
Product Description
NSE Mouse mAb is an ELK Biotechnology antibody for research workflows involving NSE.
Background
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Jurkat, 3) 293T cell lysates with NSE mAb diluted at 1:3,000. | IHC staining of Human small cell carcinoma of lung tissue with NSEmouse mAb(13E2) diluted at 1:200. |
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Key Facts
| Target | NSE |
| Also known as | ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase |
| Concentration | 1mg/mL |
| Working concentration | WB 1:2,000 IHC 1:200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 47kDa |
| Cellular localization | Cytoplasm. Cell membrane. |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:2,000 IHC 1:200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:2 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.