Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92417

GFAP Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

GFAP Mouse mAb from ELK Biotechnology Read more ›

GFAP Mouse mAb
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GFAP Mouse mAb

Product Description

GFAP Mouse mAb is a research-use antibody from ELK Biotechnology for studying GFAP in WB, IHC workflows with listed reactivity for Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

GFAP is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Additional antibody information

Validation evidence captions Western blot analysis of Rat Brain Tissue with GFAP mAb diluted at 1:5,000. | Western blot analysis of Mouse Brain Tissue with GFAP mAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using GFAP(EM1059)Mouse mAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: GFAP
Collaborator ELK Biotechnology
Partner model EM1059
Target GFAP
Research areasAntibody Research
Reactivity Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:2,000-5,000 IHC 1:200-500
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Rat WB 1:2
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
GFAP
Also known as
Glial Fibrillary Acidic Protein ;Intermediate Filament Protein
Concentration
1mg/mL
Working concentration
WB 1:2,000-5,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
45kDa
Cellular localization
Cytoplasm. Associated with intermediate filaments
Dilution range
WB 1:2,000-5,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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