GFAP Mouse mAb
GFAP Mouse mAb from ELK Biotechnology Read more ›
Product Description
GFAP Mouse mAb is an ELK Biotechnology antibody for research workflows involving GFAP.
Background
GFAP is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Additional antibody information
| Validation evidence captions | Western blot analysis of Rat Brain Tissue with GFAP mAb diluted at 1:5,000. | Western blot analysis of Mouse Brain Tissue with GFAP mAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using GFAP（EM1059）Mouse mAb diluted at 1:500. |
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Key Facts
| Target | GFAP |
| Also known as | Glial Fibrillary Acidic Protein ;Intermediate Filament Protein |
| Concentration | 1mg/mL |
| Working concentration | WB 1:2,000-5,000 IHC 1:200-500 |
| Species reactivity | Rat, Mouse |
| Observed band | 45kDa |
| Cellular localization | Cytoplasm. Associated with intermediate filaments |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:2,000-5,000 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Rat | WB 1:2 | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.