Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92411

p53 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

p53 Mouse mAb from ELK Biotechnology Read more ›

p53 Mouse mAb
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p53 Mouse mAb

Product Description

p53 Mouse mAb is a research-use antibody from ELK Biotechnology for studying p53 in WB, IHC, IF workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

Additional antibody information

Validation evidence captions Western blot analysis of 293T, with p53 mAb diluted at 1) 1:1,000 2) 1:2,000 | IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb(6C4)diluted at 1:200. | IF analysis of Hela with EM1052(Left) and DAPI (Right) diluted at 1:100.

Key Facts

Product typePrimary Antibody Target: p53
Collaborator ELK Biotechnology
Partner model EM1052
Target p53
Research areasAntibody Research
Reactivity Human
Applications WB,IHC,IF
ConjugationUnconjugated
Dilution rangeWB 1:2,000 IHC 1:200 IF 1:100-200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:2
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
p53
Also known as
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Concentration
1mg/mL
Working concentration
WB 1:2,000 IHC 1:200 IF 1:100-200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHCIF
Immunogen
Synthetic Peptide
Observed band
53kDa
Cellular localization
Cytoplasm; Cytoplasm. Nucleus
Dilution range
WB 1:2,000 IHC 1:200 IF 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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