ABM92411
p53 Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
p53 Mouse mAb from ELK Biotechnology Read more ›
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Product Description
p53 Mouse mAb is an ELK Biotechnology antibody for research workflows involving p53.
Background
p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.
Additional antibody information
| Validation evidence captions | Western blot analysis of 293T, with p53 mAb diluted at 1) 1:1,000 2) 1:2,000 | IHC staining of Human colon cancer tissue paraffin-embedded with p53 mouse mAb（6C4）diluted at 1:200. | IF analysis of Hela with EM1052(Left) and DAPI (Right) diluted at 1:100. |
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Key Facts
Product type
Primary Antibody›
Target: p53
| Target | p53 |
| Also known as | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:2,000 IHC 1:200 IF 1:100-200 |
| Species reactivity | Human |
| Observed band | 53kDa |
| Cellular localization | Cytoplasm; Cytoplasm. Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:2,000 IHC 1:200 IF 1:100-200 |
| Immunogen | Synthetic Peptide |
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Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:2 | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
p53
Also known as
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Concentration
1mg/mL
Working concentration
WB 1:2,000 IHC 1:200 IF 1:100-200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHCIF
Immunogen
Synthetic Peptide
Observed band
53kDa
Cellular localization
Cytoplasm; Cytoplasm. Nucleus
Dilution range
WB 1:2,000 IHC 1:200 IF 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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