HSP70 Mouse mAb
HSP70 Mouse mAb from ELK Biotechnology Read more ›
Product Description
HSP70 Mouse mAb is an ELK Biotechnology antibody for research workflows involving HSP70.
Background
The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Mouse Brain, with HSP70 mAb diluted at 1:2,000. | Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), using HSP70 diluted at 1:2,000. | IF analysis of Hela with EM1042(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using HSP70 （EM1042）Mouse mAb diluted at 1:500. |
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Key Facts
| Target | HSP70 |
| Also known as | Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IF 1:100-200 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 70kDa |
| Cellular localization | Cytoplasm |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:1,000-2,000 IF 1:100-200 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.