ABM92397
Histone H3 Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
Histone H3 Mouse mAb from ELK Biotechnology Read more ›
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Product Description
Histone H3 Mouse mAb is an ELK Biotechnology antibody for research workflows involving Histone H3.
Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with EM1037 diluted at 1:5,000. | IF analysis of Hela with DAPI (Left) and EM1037(Right) diluted at 1:200. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1037 1:2,000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000 |
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Key Facts
Product type
Primary Antibody›
Target: Histone H3
| Target | Histone H3 |
| Also known as | H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:2,000-5,000 IP 1:200 IF 1:200 |
| Species reactivity | Human, Mouse, Rat, Yeast |
| Observed band | 15kDa |
| Cellular localization | Nucleus,Chromosome |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFIP |
| Dilution range | WB 1:2,000-5,000 IP 1:200 IF 1:200 |
| Immunogen | Recombinant Protein |
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Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Yeast |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:2 | — |
| Mouse | — | — |
| Rat | — | — |
| Yeast | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IP
| Species | Dilution | Notes |
|---|
Specifications
Target
Histone H3
Also known as
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Concentration
1mg/mL
Working concentration
WB 1:2,000-5,000 IP 1:200 IF 1:200
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Mouse, Rat, Yeast
Tested applications
WBIHCIFIP
Immunogen
Recombinant Protein
Observed band
15kDa
Cellular localization
Nucleus,Chromosome
Dilution range
WB 1:2,000-5,000 IP 1:200 IF 1:200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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