ABM92394
α-tubulin Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
α-tubulin Mouse mAb from ELK Biotechnology Read more ›
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Product Description
α-tubulin Mouse mAb is an ELK Biotechnology antibody for research workflows involving α-tubulin.
Background
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with EM1034 diluted at 1:5,000. | Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using α tubulin （EM1034）Mouse mAb diluted at 1:500. | 1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1034 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000 | IF analysis of Hela with EM1034(Left) and DAPI (Right) diluted at 1:100. |
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Key Facts
Product type
Primary Antibody›
Target: α-tubulin
| Target | α-tubulin |
| Also known as | alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 52kDa |
| Cellular localization | Cytoplasm,Cytoskeleton,Microtubule |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFIP |
| Dilution range | WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IP
| Species | Dilution | Notes |
|---|
Specifications
Target
α-tubulin
Also known as
alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain
Concentration
1mg/mL
Working concentration
WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHCIFIP
Immunogen
Recombinant Protein
Observed band
52kDa
Cellular localization
Cytoplasm,Cytoskeleton,Microtubule
Dilution range
WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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