COX IV Mouse mAb
COX IV Mouse mAb from ELK Biotechnology Read more ›
Product Description
COX IV Mouse mAb is an ELK Biotechnology antibody for research workflows involving COX IV.
Background
The enzyme cytochrome c oxidase or Complex IV, EC 1.9.3.1) is a large transmembrane protein complex found in bacteria and the mitochondrion.It is the last enzyme in the respiratory electron transport chain of mitochondria (or bacteria) located in the mitochondrial (or bacterial) membrane. It receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In the process, it binds four protons from the inner aqueous phase to make water, and in addition translocates four protons across the membrane, helping to establish a transmembrane difference of proton electrochemical potential that the ATP synthase then uses to synthesize ATP.
Additional antibody information
| Validation evidence captions | Western blot analysis of Hela with COX IV Mouse mAb diluted at 1）1:2,000 2）1:5,000 | Western blot analysis of 1) HepG2, 2) 293T, 3) Rat Heart, 4) Mouse Heart, 5) Mouse Muscle tissue with COX IV Mouse mAb) diluted at 1:3,000. | Western blot analysis of Hela with COX IV Mouse mAb diluted at 1:3,000. | IF analysis of Hela with EM1032(Left) and DAPI (Right) diluted at 1:100. |
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Key Facts
| Target | COX IV |
| Also known as | COX 4, Cox4a, COX4B, COXIV, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, MGC105470, dj857M17.2, Cytochrome c oxidase subunit IV |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-3,000 IF 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 15kDa |
| Cellular localization | Membrane,Mitochondrion,Mitochondrion inner membrane |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIF |
| Dilution range | WB 1:1,000-3,000 IF 1:100-200 |
| Immunogen | Recombinant Protein |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.