COX IV Mouse mAb from ELK Biotechnology Read more ›
COX IV Mouse mAb is a research-use antibody from ELK Biotechnology for studying COX IV in WB, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The enzyme cytochrome c oxidase or Complex IV, EC 1.9.3.1) is a large transmembrane protein complex found in bacteria and the mitochondrion.It is the last enzyme in the respiratory electron transport chain of mitochondria (or bacteria) located in the mitochondrial (or bacterial) membrane. It receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In the process, it binds four protons from the inner aqueous phase to make water, and in addition translocates four protons across the membrane, helping to establish a transmembrane difference of proton electrochemical potential that the ATP synthase then uses to synthesize ATP.
| Validation evidence captions | Western blot analysis of Hela with COX IV Mouse mAb diluted at 1）1:2,000 2）1:5,000 | Western blot analysis of 1) HepG2, 2) 293T, 3) Rat Heart, 4) Mouse Heart, 5) Mouse Muscle tissue with COX IV Mouse mAb) diluted at 1:3,000. | Western blot analysis of Hela with COX IV Mouse mAb diluted at 1:3,000. | IF analysis of Hela with EM1032(Left) and DAPI (Right) diluted at 1:100. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1032 |
| Target | COX IV |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-3,000 IF 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant Protein |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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