PCNA Mouse mAb
PCNA Mouse mAb from ELK Biotechnology Read more ›
Product Description
PCNA Mouse mAb is an ELK Biotechnology antibody for research workflows involving PCNA.
Background
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Additional antibody information
| Validation evidence captions | Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000. | IHC staining of Human breast cancer tissue paraffin-embedded with PCNA mouse mAb（12D10）diluted at 1:200 |
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Key Facts
| Target | PCNA |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 IHC 1:200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 29kDa |
| Cellular localization | Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:5,000 IHC 1:200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.