S-Tag Mouse mAb from ELK Biotechnology Read more ›
S-Tag Mouse mAb is a research-use antibody from ELK Biotechnology for studying S-Tag in WB workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.The amino acid sequence of the S-tag is: KETAAAKFERQHMDS conjugated to KLH. S- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.
| Validation evidence captions | 2ug S-Tag fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 |
|---|
| Collaborator | ELK Biotechnology |
| Partner model | EM1016 |
| Target | S-Tag |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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