S-Tag Mouse mAb
S-Tag Mouse mAb from ELK Biotechnology Read more ›
Product Description
S-Tag Mouse mAb is an ELK Biotechnology antibody for research workflows involving S-Tag.
Background
S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.The amino acid sequence of the S-tag is: KETAAAKFERQHMDS conjugated to KLH. S- Tag antibody can recognize C-terminal, internal, and N-terminal S-tagged proteins.
Additional antibody information
| Validation evidence captions | 2ug S-Tag fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 |
|---|
Key Facts
| Target | S-Tag |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 |
| Species reactivity | Epitope tag |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:5,000 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 target type| Target type | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Target type | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.