V5-Tag Mouse mAb from ELK Biotechnology Read more ›
V5-Tag Mouse mAb is a research-use antibody from ELK Biotechnology for studying V5-Tag in WB, IF, IP workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein.
| Validation evidence captions | IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G ag | IF analysis of 293 cells transfected with a V5-tag protein, using ELKbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5) | 1ug V5 fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1004 |
| Target | V5-Tag |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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