ABM92369
His-Tag Mouse mAb
ISO 9001|ABMIUM Verified™|Antibody
His-Tag Mouse mAb from ELK Biotechnology Read more ›
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Product Description
His-Tag Mouse mAb is an ELK Biotechnology antibody for research workflows involving His-Tag.
Background
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Additional antibody information
| Validation evidence captions | IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Prote | IF analysis of 293 cells transfected with a His-tag protein, using ELKbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His) | 2ug His fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 | 1、Multi-Tag Recombinant protein 2、293T transfected with His recombinant protein 3、Insect cell trandfected with His recombinant protein |
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Key Facts
Product type
Primary Antibody›
Target: His-Tag
| Target | His-Tag |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Species reactivity | Epitope tag |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIFIP |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen | Synthetic Peptide |
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Citations
ISO 9001
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Reactivity & Application Validation
1 target type| Target type | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
ABMIUM Validated™
WB
| Target type | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
ABMIUM Validated™
IF
| Target type | Dilution | Notes |
|---|
ABMIUM Validated™
IP
| Target type | Dilution | Notes |
|---|
Specifications
Target
His-Tag
Concentration
1mg/mL
Working concentration
WB 1:5,000 IP 1:200 IF 1:1,000
Form / buffer
Antibody solution
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Epitope tag
Tested applications
WBIFIP
Immunogen
Synthetic Peptide
Dilution range
WB 1:5,000 IP 1:200 IF 1:1,000
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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