HA-Tag Mouse mAb
HA-Tag Mouse mAb from ELK Biotechnology Read more ›
Product Description
HA-Tag Mouse mAb is an ELK Biotechnology antibody for research workflows involving HA-Tag.
Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.
Additional antibody information
| Validation evidence captions | IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with HA-tag fusion protein 3、IP(untransfted 293+anti-HA mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G ag | IF analysis of 293 cells transfected with a HA-tag protein, using ELKbio anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA) | 0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000. |
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Key Facts
| Target | HA-Tag |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Species reactivity | Epitope tag |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIFIP |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
1 target type| Target type | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Target type | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
| Target type | Dilution | Notes |
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| Target type | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.