HA-Tag Mouse mAb from ELK Biotechnology Read more ›
HA-Tag Mouse mAb is a research-use antibody from ELK Biotechnology for studying HA-Tag in WB, IF, IP workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.
| Validation evidence captions | IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with HA-tag fusion protein 3、IP(untransfted 293+anti-HA mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G ag | IF analysis of 293 cells transfected with a HA-tag protein, using ELKbio anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA) | 0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1002 |
| Target | HA-Tag |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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