Human KDM2B(Lysine (K) specific demethylase 2B) ELISA Kit

Human KDM2B(Lysine (K) specific demethylase 2B) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring KDM2B in Serum, Plasma, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KDM2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KDM2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KDM2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KDM2B in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include [Histone-H3]-lysine-36 demethylase 1B; CXXC-type zinc finger protein 2; CXXC2; F box and leucine rich repeat protein 10; F box protein FBL10; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; Fbl10; FBXL10; JEMMA (Jumonji domain EMSY interactor methyltransferase motif)..