Mouse ANA(anti-nuclear Antibody) ELISA Kit from ELK Biotechnology Read more ›
Mouse ANA(anti-nuclear Antibody) ELISA Kit is a research-use antibody from ELK Biotechnology for studying ANA in ELISA workflows with listed reactivity for Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ANA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ANA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ANA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ANA in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include Anti nuclear Antibody.
| Collaborator | ELK Biotechnology |
| Partner model | ELK9820 |
| Target | ANA |
| Research areas | Biochemical research |
| Reactivity | Mouse |
| Applications | ELISA |
| Assay type | Sandwich |
| Sample types | Serum,Plasma,Tissue homogenates,Cell lysates,Cell culture supernatant,Other biological fluids |
| Detection range | 1.57-100 ng/mL |
| Sensitivity | 0.58 ng/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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